Biocatalysis

Dirk Tischler

Mei 2025 · Methods in Enzymology Boek 714 · Academic Press

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Biocatalysis, Volume 714 provides a wide range of themes dealing with the identification and application of biocatalysts. This includes various formats such as whole-cell or cell-free biocatalysis as well as immobilized variants. Specific chapters in this new release include Biocatalysis: How to select the proper mode of application, Documentation in biocatalysis and data handling, Mining metagenomes from extremophiles as resource for novel glycoside hydrolases for industrial applications, Functional Metaproteomics, Sequence-function relation for the prediction of enzyme properties: A case study on flavin-dependent oxidases, P450 monooxygenase in whole-cell format, and more.Additional sections cover Regio- and Stereospecific oxidation based on di-iron monooxygenases producing whole cells, Recombinant enzyme expression and targeted mutagenesis in Aromatoleum species, C.necator as a model organism for CO2-based biotechnology, Cupriavidus in whole-cell biocatalysis, Whole-cell biocatalysis with Myxobacteria, Streptomyces for natural product formation: Targeted mutagenesis in PKS, Reductive Amination: Methods for cell-free and whole-cell biocatalysis, Challenges and good practices on transaminase-catalysed synthesis of optically pure amines, W-enzymes in biocatalysis: Chances and difficulties for the user, Atroposelective biocatalysis employing ADHs, Applications of alcohol oxidases, and much more. - Provides guidance on how to identify, produce, describe, and apply biocatalysts, including documentation - Includes broad perspectives on biocatalysis, including its advantages as well as its hurdles - Presents a plethora of applications of a diverse set of enzyme-based catalysis

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Dirk Tischler studied Applied Natural Science with focal points on Environmental Microbiology and Biotechnology as well as Environmental Analytics at TU Bergakademie Freiberg (Germany) and graduated in 2007. He continued in Freiberg with his doctoral studies on styrene monooxygenases and related enzymes and completed his dissertation with distinction in 2012. For this he was awarded with two pre-doctoral scholarships from the Deutsche Bundesstiftung Umwelt and Fulbright and he had two international research stays in Biochemistry Laboratories with Willem van Berkel at Wageningen University and George Gassner at San Francisco State University. The doctoral thesis was awarded with two prizes. He continued as a group leader in the field of industrial biotechnology with emphasis on the prediction of biocatalysts and pathways from (meta)genomes of soil bacteria, especially actinobacteria. This includes three prestigious grants for his group projects GETGEOWEB (ESF), BakSolEx (BMBF), and ChemBioCat (NRW). 2018 he became W1 Professor and was tenured in 2019 to Full Professor for Microbial Biotechnology at the Ruhr University Bochum. He focuses on the identification and application of novel biocatalysts, mostly related to redox biochemistry.

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