Plant Transposable Elements
O. Nelson
āļ.āļ. 2012 · Basic Life Sciences āļŦāļāļąāļāļŠāļ·āļāđāļĨāđāļĄāļāļĩāđ 47 · Springer Science & Business Media
eBook
404
āļŦāļāđāļē
reportāļāļ°āđāļāļāđāļĨāļ°āļĢāļĩāļ§āļīāļ§āđāļĄāđāđāļāđāļĢāļąāļāļāļēāļĢāļāļĢāļ§āļāļŠāļāļāļĒāļ·āļāļĒāļąāļ Â āļāļđāļāđāļāļĄāļđāļĨāđāļāļīāđāļĄāđāļāļīāļĄ
āđāļāļĩāđāļĒāļ§āļāļąāļ eBook āđāļĨāđāļĄāļāļĩāđ
Transposon tagging can work. Even though most of our understand ing about the factors that contribute to a successful tagging experiment has been accumulated from a limited number of experiments using different transposable elements in different genetic backgrounds, it is still possible to draw some conclusions regarding the best experimental strategies for gene tagging. In our experience, Spm has proved to be a good element for transposon tagging. The frequency of recovering mutable alleles in duced by Spm is not significantly different from that for Ac-Ds or for Mu 6 (summarized in Ref. 22) and varies from about 10- to 10=zr:-8pm has the unique advantage, however, in that all of the members of thiSfumily that have been examined thus far are homologous to each other at the DNA level. Therefore, by combining molecular analysis with genetic segre gation, it is possible to identify and isolate alleles that are due to insertions of either autonomous or nonautonomous Spm elements. There are definite steps one can take to increase the chances of de tecting a transposition into the gene of interest. The most important step is to select a genetic background in which the desired phenotype will be easy to screen. If the phenotype is not likely to be mutable, then tester lines should be constructed so as to contain flanking markers that can aid in subsequent segregation analyses.
āđāļŦāđāļāļ°āđāļāļ eBook āļāļĩāđ
āđāļŠāļāļāļāļ§āļēāļĄāđāļŦāđāļāļāļāļāļāļļāļāđāļŦāđāđāļĢāļēāļĢāļąāļāļĢāļđāđ
āļāđāļāļĄāļđāļĨāđāļāļāļēāļĢāļāđāļēāļ
āļŠāļĄāļēāļĢāđāļāđāļāļāđāļĨāļ°āđāļāđāļāđāļĨāđāļ
āļāļīāļāļāļąāđāļāđāļāļ Google Play Books āļŠāļģāļŦāļĢāļąāļ Android āđāļĨāļ° iPad/iPhone āđāļāļāļāļ°āļāļīāļāļāđāđāļāļĒāļāļąāļāđāļāļĄāļąāļāļīāļāļąāļāļāļąāļāļāļĩāļāļāļāļāļļāļ āđāļĨāļ°āļāđāļ§āļĒāđāļŦāđāļāļļāļāļāđāļēāļāđāļāļāļāļāļāđāļĨāļāđāļŦāļĢāļ·āļāļāļāļāđāļĨāļāđāđāļāđāļāļļāļāļāļĩāđ
āđāļĨāđāļāļāđāļāļāđāļĨāļ°āļāļāļĄāļāļīāļ§āđāļāļāļĢāđ
āļāļļāļāļāļąāļāļŦāļāļąāļāļŠāļ·āļāđāļŠāļĩāļĒāļāļāļĩāđāļāļ·āđāļāļāļēāļ Google Play āđāļāļĒāđāļāđāđāļ§āđāļāđāļāļĢāļēāļ§āđāđāļāļāļĢāđāđāļāļāļāļĄāļāļīāļ§āđāļāļāļĢāđāđāļāđ
eReader āđāļĨāļ°āļāļļāļāļāļĢāļāđāļāļ·āđāļāđ
āļŦāļēāļāļāđāļāļāļāļēāļĢāļāđāļēāļāļāļāļāļļāļāļāļĢāļāđ e-ink āđāļāđāļ Kobo eReader āļāļļāļāļāļ°āļāđāļāļāļāļēāļ§āļāđāđāļŦāļĨāļāđāļĨāļ°āđāļāļāđāļāļĨāđāđāļāļĒāļąāļāļāļļāļāļāļĢāļāđāļāļāļāļāļļāļ āđāļāļĢāļāļāļģāļāļēāļĄāļ§āļīāļāļĩāļāļēāļĢāļāļĒāđāļēāļāļĨāļ°āđāļāļĩāļĒāļāđāļāļĻāļđāļāļĒāđāļāđāļ§āļĒāđāļŦāļĨāļ·āļāđāļāļ·āđāļāđāļāļāđāļāļĨāđāđāļāļĒāļąāļ eReader āļāļĩāđāļĢāļāļāļĢāļąāļ
