Green Synthesis of Silver Nanoparticles using Stingless bee (Trigona iridipennis Smith) honey and evaluation of their antibacterial activity

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Nanotechnology is an emerging field of science. It has increased applications in diverse area for the development of new materials at nanoscale levels. Synthesis of nanoparticles using biological methods is referred as greener synthesis of nanoparticles. Green synthesis provides advancement over chemical and physical method as it is cost effective, environment friendly, and safe for human therapeutic use. Stingless bees are highly social (eusocial) insects which populated the tropical earth 65 million years ago longer than honey bees. Among the most common uses of stingless bee honey are to treat stomach disturbance, cough, tonsillitis, sore throat, stomach and intestinal ulcers, cold, disease of the mouth, mucus membrane, and as a wound dressing due to its antimicrobial activity. Stingless bees honey were used to for the green synthesis of silver nanoparticles. Antimicrobial activity of the green synthesised nanoparticles were tested used agar diffusion method against Escherichia coli (E. coli), Pseudomonas aeruginosa, Staphylococcus aureus, Salmonella typhi and Klebsiella pneumoniae. The results showed that stingless bee honey could be effectively used for the synthesis of silver nanoparticle. The synthesized silver nanoparticles shows antibacterial activity on both Gram positive and Gram negative bacteria. This biosynthesis of nanoparticles is cost efficient, pollutant free and simpler to synthesize.

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