This practical guide, developed by TDR Global, focuses on enhancing health research mentorship in low- and middle-income countries (LMICs). It addresses the unique challenges faced by researchers in these settings and provides tools and open-access resources to support mentorship programs. The guide emphasizes the importance of bridging the generational gap in science through mentorship, which is seen as crucial for nurturing research impact and strengthening both individual and institutional capacities. It is informed by proposals from 40 countries and systematic reviews, offering pathways to mentorship tailored for LMICs. The intended audience includes research institutions and professionals in LMICs seeking to build effective mentorship programs.